Optical Imaging Center Erlangen (OICE)

The OICE offers and coordinates a direct access to modern microscopy technology. The involvement of the OICE within the CRC 1181 allows scientists to obtain the possibility to get a more detailed insight into the molecular mechanisms and processes from a macroscopic to nanoscopic scale.

Technics and methods

Modalities	IVIS Spectrum & µCT	Intravital Microscope	Spinning Disc LSM	Confocal LSM	STED / RESOLFT	iSCAT / STORM
Technics	Molecular and anatomical views of complex biological systems high resolution (up to 4.5 micrometer voxel size) Multimodal 3D Imaging with parallel tomographic imaging of fluoresence and luminesence signals Longitudinal studies to  monitoring disease progression in vivo Compensation possibility for motion artefacts (i.g. of breath and cardiac action)	High resolution multiphoton imaging Spectra Physics Insight DeepSee Dual (680 – 1300 nm) Laser – high penetration depth 2 GaAsP NDDs in objective halter Investigation of different tissue in vivo high penetration depth	High resolution confocal imaging Longtudial living cell microscopy normoxic and hypoxischen environmental factors Fast mulit-camera -system   Multichannel fluorescence	High resolution confocal imaging 8 laser excitation wave lengths (405 ~ 647 nm) 6 detector units (3 PMTs, 2 HyDs, 1 TMP) Extremly fast point scanner ( max. 8.000 Hz) Normoxic living cell microscopy	Super high resolution fluorescence microscopy Fixed samples and living cell microscopy (normoxic) 3 canal STED 3 APD detectors 3D STED compedent RESOLFT longtudial living cell microscopy	Super high resolution imaging No labelling needed single-molecule imaging single-molecule tracking
Resolution	macro 1µm	micro 200nm	micro 200nm	micro 160nm	nano 2D 10 x 10 x 500 nm oder 3D 90 x 90 x 90 nm	nano 5nm, Localisation precision < 1 nm

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